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          物競編號 0KHQ
          分子式 暫無
          分子量 暫無
          標簽 Terminal deoxynu-cleotidyl transferase, Nucleosidetriphosphate, DNA deoxynucleotidyl exotransferase

          編號系統(tǒng)

          CAS號:9027-67-2

          MDL號:MFCD00166324

          EINECS號:暫無

          RTECS號:暫無

          BRN號:暫無

          PubChem號:暫無

          物性數據

          1.      性狀:冷凍干燥含氯化鉀的固體

          2.       密度(g/mL,25):未確定

          3.       相對蒸汽密度(g/mL,空氣=1):未確定

          4.       熔點(oC):未確定

          5.       沸點(oC,常壓):未確定

          6.       沸點(oC,5.2kPa):未確定

          7.       折射率:未確定

          8.       閃點(oC):未確定

          9.       比旋光度(o):未確定

          10.    自燃點或引燃溫度(oC):未確定

          11.    蒸氣壓(kPa,20oC):未確定

          12.    飽和蒸氣壓(kPa,60oC):未確定

          13.    燃燒熱(KJ/mol):未確定

          14.    臨界溫度(oC):未確定

          15.    臨界壓力(KPa):未確定

          16.    油水(辛醇/水)分配系數的對數值:未確定

          17.    爆炸上限(%,V/V):未確定

          18.    爆炸下限(%,V/V):未確定

          19.    溶解性:未確定

          毒理學數據

          暫無

          生態(tài)學數據

          通常對水是不危害的,若無政府許可,勿將材料排入周圍環(huán)境。

          分子結構數據

          暫無

          計算化學數據

          暫無

          性質與穩(wěn)定性

          Analysis Note Activity assay: 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C.

          Biochem/physiol Actions Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3 overhangs, but procedures have been developed to label blunt ends or 3-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present. Each reaction mixture needs to be optimized. The enzyme is commonly used to add complementary homopolymer tails to vector and insert DNA for construction of recombinant DNA in vitro.

          Features and Benefits ? Add homopolymers to vectors, inserts and cDNA for cloning

          ? Label the 3-end of double- and single-stranded DNA with non-radioactive and radioactive labels

          ? Carry out in vitro mutagenesis by adding single nucleotides to DNA

          ? Use in TUNEL assays with deoxyuridine triphosphate (dUTP) conjugated with a fluorescent chromophore or biotin to label nicked DNA in apoptotic cells

           

          Unit Definition One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer.

          Physical form Solution in 50 mM potassium phosphate, pH 7.4, 1 mM 2-mercaptoethanol and 50% glycerol (v/v)

          貯存方法

          充氬-20°C密閉,避光,通風干燥處

          合成方法

          暫無

          用途

          生化研究

          安全信息

          危險運輸編碼:暫無

          危險品標志:暫無

          安全標識:暫無

          危險標識:暫無

          文獻

          暫無

          備注

          暫無

          表征圖譜